Chromogranin A anticorps

N° du produit ABIN3024551

Cet anticorps Souris Monoclonal détecte spécifiquement Chromogranin A dans IF, FACS et IHC (p). Il présente une réactivité envers Humain, Rat, Souris, Singe et Porc.

Aperçu rapide pour Chromogranin A anticorps (ABIN3024551)

Antigène: Chromogranin A (CHGA)

Reactivité: Humain, Rat, Souris, Singe, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Chromogranin A est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: LK2H10

Détail du produit anti-Chromogranin A anticorps

Aucune reactivité croisée: Cobaye, Mouton, Lapin

Purification: Protein G purified monoclonal antibody

Immunogène: Human pheochromocytoma cells were used as the immunogen for this Chromogranin A antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Chromogranin A antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Chromogranin A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur Chromogranin A

Antigène: Chromogranin A (CHGA)

Autre désignation: Chromogranin A

Sujet: Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong chromogranin A antibody staining and absence of keratin antibody staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.

ID gène: 1113

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling